Anticorps Recombinant de lapin anti-DeltaN p63

DeltaN p63 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251209D11

N° de cat : 86154-11-PBS

Synonymes

p63, TP63, CUSP, TP 63, Transformation-related protein 63

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Galerie de données de validation

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  WB analysis using 86154-11-RR (same clone as 86154-11-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86154-11-RR (TP63 DeltaN(P40) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86154-11-PBS in a different storage buffer formulation.

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  WB analysis using 86154-11-RR (same clone as 86154-11-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86154-11-RR (TP63 DeltaN(P40) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86154-11-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 86154-11-RR (same clone as 86154-11-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using TP63 DeltaN(P40) antibody (86154-11-RR, Clone: 251209D11 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86154-11-PBS in a different storage buffer formulation.

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Informations sur le produit

86154-11-PBS cible DeltaN p63 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: tumor protein p63
• Masse moléculaire calculée: 66 kDa
• Poids moléculaire observé: 68-75 kDa
• Numéro d’acquisition GenBank: NM_001114980
• Symbole du gène: TP63
• Identification du gène (NCBI): 8626
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DeltaN p63 (ΔNp63) is a major protein isoform encoded by the TP63 gene. The TP63 gene is a member of the p53 gene family, which also includes p53 and p73. The key defining feature of DeltaN p63 (ΔNp63) is the absence of the full N-terminal transactivation domain. However, it retains the central DNA-binding domain (DBD) and the C-terminal oligomerization domain (OD). The α-isoform also has a sterile alpha motif (SAM) domain and a transcriptional inhibitory domain (TID). DeltaN p63 isoforms can act as dominant-negative inhibitors of p53 and TAp63 by competing for DNA binding sites or forming non-functional dimers. DeltaN p63 is highly expressed in basal layers of epithelial tissues and is essential for the maintenance and proliferation of epithelial stem cells. It plays a pivotal role in epidermal development, stratification, and regeneration.

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