Anticorps Recombinant de lapin anti-DeltaN p63

DeltaN p63 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

251209D11

N° de cat : 86154-11-RR

Synonymes

p63, TP63, CUSP, TP 63, Transformation-related protein 63

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Galerie de données de validation

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  WB analysis using 86154-11-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86154-11-RR (TP63 DeltaN(P40) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86154-11-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86154-11-RR (TP63 DeltaN(P40) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 86154-11-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using TP63 DeltaN(P40) antibody (86154-11-RR, Clone: 251209D11 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules A431, cellules HaCaT, cellules PC-12, cellules U-937, tissu cutané de souris
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86154-11-RR cible DeltaN p63 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: tumor protein p63
• Masse moléculaire calculée: 66 kDa
• Poids moléculaire observé: 68-75 kDa
• Numéro d’acquisition GenBank: NM_001114980
• Symbole du gène: TP63
• Identification du gène (NCBI): 8626
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DeltaN p63 (ΔNp63) is a major protein isoform encoded by the TP63 gene. The TP63 gene is a member of the p53 gene family, which also includes p53 and p73. The key defining feature of DeltaN p63 (ΔNp63) is the absence of the full N-terminal transactivation domain. However, it retains the central DNA-binding domain (DBD) and the C-terminal oligomerization domain (OD). The α-isoform also has a sterile alpha motif (SAM) domain and a transcriptional inhibitory domain (TID). DeltaN p63 isoforms can act as dominant-negative inhibitors of p53 and TAp63 by competing for DNA binding sites or forming non-functional dimers. DeltaN p63 is highly expressed in basal layers of epithelial tissues and is essential for the maintenance and proliferation of epithelial stem cells. It plays a pivotal role in epidermal development, stratification, and regeneration.

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