Anticorps Polyclonal de lapin anti-Destrin

Destrin Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10962-2-PBS

Synonymes

DSTN, DSN, ADF, Actin-depolymerizing factor, ACTDP

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Galerie de données de validation

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  WB analysis using 10962-2-AP (same clone as 10962-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10962-2-AP (Destrin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10962-2-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 10962-2-AP (same clone as 10962-2-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 10962-2-AP (Destrin antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10962-2-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 10962-2-AP (same clone as 10962-2-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Destrin antibody (10962-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10962-2-PBS in a different storage buffer formulation.

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Informations sur le produit

10962-2-PBS cible Destrin dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Destrin Protéine recombinante Ag1402
• Nom complet: destrin (actin depolymerizing factor)
• Masse moléculaire calculée: 19 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC009477
• Symbole du gène: Destrin
• Identification du gène (NCBI): 11034
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Destrin (also known as actin depolymerization factor, ADF) is an actin-binding protein contributing to cytoskeletal dynamics by promoting rapid actin filament disassembly. By regulating actin cytoskeletal reorganization it plays an important role in the formation of filopodia, which further promotes tumor cell invasion and metastasis. Destrin has been reported to be overexpressed in primary colon cancer.

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