Anticorps Recombinant de lapin anti-DFNA5/GSDME

DFNA5/GSDME Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251415B1

N° de cat : 82955-2-PBS

Synonymes

Dfna5, Dfna5h, EG14210, Fin15, Gasdermin-E

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Galerie de données de validation

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  WB analysis using 82955-2-RR (same clone as 82955-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82955-2-RR (Dfna5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82955-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP02686-1

  The mean DFNA5/GSDME concentration was determined to be 85.78 ng/mL in J744A.1 cell extract based on a 1.3 mg/mL extract load, 16.86 ng/mL in mouse brain tissue extract based on a 7.0 mg/mL extract load and 10.69 ng/mL in rat brain tissue extract based on a 8.2 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02686-1

  Sandwich ELISA standard curve of MP02686-1, Mouse DFNA5/GSDME Recombinant Matched Antibody Pair - PBS only. 82955-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag33622. 82955-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL

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  Affinity and Kinetic Characterization of 82955-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82955-2-RR against Mouse DFNA5/GSDME were performed. The affinity constant is below 1 pM.

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Informations sur le produit

82955-2-PBS cible DFNA5/GSDME dans les applications de WB, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DFNA5/GSDME Protéine recombinante Ag33622
• Nom complet: deafness, autosomal dominant 5 (human)
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: BC132303
• Symbole du gène: DFNA5/GSDME
• Identification du gène (NCBI): 54722
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain.

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