Anticorps Recombinant de lapin anti-Di-Methyl-Histone H4 (Arg3)

Di-Methyl-Histone H4 (Arg3) Recombinant Antibody for WB, Dot Blot, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Dot Blot, ELISA

Conjugaison

Non conjugué

CloneNo.

1E21

N° de cat : 82859-1-RR

Synonymes

Di-Methyl-Histone H4, Di-Methyl Histone-H4 Arg3, Di-Methyl Histone H4 Arg3, Di-Methyl Histone H4 (Arg3), Di Methyl Histone H4

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Galerie de données de validation

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  WB analysis using 82859-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82859-1-RR (Di-Methyl-Histone H4 (Arg3) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 82859-1-RR

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 82859-1-RR (Di-Methyl-Histone H4 (Arg3) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  Dot Blot experiment of peptide using 82859-1-RR

  Dot blot analysis was used to confirm the specificity of 82859-1-RR Di-Methyl-Histone H4 (Arg3) antibody. Peptides were spotted onto NC and probed with antibody at 5 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.

• 

  Dot Blot experiment of peptide using 82859-1-RR

  Dot blot analysis was used to confirm the specificity of 82859-1-RR Di-Methyl-Histone H4 (Arg3) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.

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Applications testées

• Résultats positifs en WB: cellules THP-1, cellules HSC-T6, cellules NIH/3T3, tissu hépatique de souris
• Résultats positifs en Dot Blot: Peptide,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• DOT BLOT: DOT BLOT : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82859-1-RR cible Di-Methyl-Histone H4 (Arg3) dans les applications de WB, Dot Blot, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: histone cluster 1, H4a
• Poids moléculaire observé: 13 kDa
• Numéro d’acquisition GenBank: BC069654
• Symbole du gène: HIST1H4A
• Identification du gène (NCBI): 8359
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

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