Anticorps Recombinant de lapin anti-Di/Tri-Methyl-Histone H3 (Lys4)

Di/Tri-Methyl-Histone H3 (Lys4) Recombinant Antibody for WB, IHC, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR

Conjugaison

Non conjugué

CloneNo.

241942D11

N° de cat : 84908-2-PBS

Synonymes

dmk4, H3 di/trimethyl-lys4, H3 Me Lys4, H3 MeK4, H3 Methyl K4

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Galerie de données de validation

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  WB analysis using 84908-2-RR (same clone as 84908-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84908-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 84908-2-RR (same clone as 84908-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84908-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 84908-2-RR (same clone as 84908-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84908-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 84908-2-RR (same clone as 84908-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84908-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 84908-2-RR (same clone as 84908-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84908-2-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 84908-2-RR (same clone as 84908-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84908-2-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 84908-2-RR (same clone as 84908-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84908-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84908-2-RR (same clone as 84908-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIST1H3A antibody (84908-2-RR, Clone: 241942D11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84908-2-PBS in a different storage buffer formulation.

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  Dot Blot experiment of peptide using 84908-2-RR (same clone as 84908-2-PBS)

  Dot blot analysis was used to confirm the specificity of 84908-2-RR Di/Tri-Methyl-Histone H3 (Lys4) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 84908-2-PBS in a different storage buffer formulation.

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  ChIP experiment of HeLa using 84908-2-RR (same clone as 84908-2-PBS)

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 10 µg of cross-linked chromatin, 3 µg of Di/Tri-Methyl-Histone H3 (Lys4) (84908-2-RR) or 3 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 84908-2-PBS in a different storage buffer formulation.

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Informations sur le produit

84908-2-PBS cible Di/Tri-Methyl-Histone H3 (Lys4) dans les applications de WB, IHC, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: histone cluster 1, H3a
• Numéro d’acquisition GenBank: BC066245
• Symbole du gène: HIST1H3A
• Identification du gène (NCBI): 8350
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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