Anticorps Polyclonal de lapin anti-DjC8

DjC8 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 32709-1-AP

Synonymes

DnaJ homolog subfamily C member 8, DNAJC8, HSPC315, HSPC331, SPF31

---

Galerie de données de validation

• 

  WB analysis using 32709-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32709-1-AP (DNAJC8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules Caco-2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

32709-1-AP cible DjC8 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DjC8 Protéine recombinante Ag38520
• Nom complet: DnaJ (Hsp40) homolog, subfamily C, member 8
• Masse moléculaire calculée: 30kDa，253aa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: NM_014280.2
• Symbole du gène: DNAJC8
• Identification du gène (NCBI): 22826
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DjC8 (C8, a member of DNAJ heat shock protein family), also known as DNAJC8, which is a highly conserved protein, belonging to heat shock protein 40(HSP40) family. The protein is expressed in many cell types, including central nervous system macrophages, cerebellar inhibitory neurons, choroid plexus epithelial cells of ventricles, oligodendrocyte precursor cells and so on. In normal tissues, the expression level of DjC8 is relatively low, but it is high in some tissues such as testis, skeletal muscle and spleen. In hepatocellular carcinoma, the high expression of DjC8 is related to poor prognosis. Knocking down DjC8 can inhibit the proliferation, migration and invasion of hepatocellular carcinoma cells, and reduce the IC50 value of sorafenib, indicating that it may be a potential therapeutic target and prognostic marker for hepatocellular carcinoma. In addition, DjC8 is regulated by TIG1 in cervical cancer and participates in glycolysis. DjC8 contains a conserved J domain, which can interact with HSP70 and activate its ATPase activity, thus participating in the folding, unfolding, transport and degradation of protein. The molecular weight of DjC8 is 30 kDa.

{{ptg:RelatedPrimaryAntibodies}}