Anticorps Polyclonal de lapin anti-Drebrin

Drebrin Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 25770-1-AP

Synonymes

D0S117E, DBN1, Drebrin, drebrin 1

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Galerie de données de validation

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  WB analysis using 25770-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25770-1-AP (Drebrin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 25770-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25770-1-AP (Drebrin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 25770-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25770-1-AP (Drebrin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 25770-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25770-1-AP (Drebrin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 25770-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25770-1-AP (Drebrin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of SH-SY5Y using 25770-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 25770-1-AP (Drebrin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 25770-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using Drebrin antibody (25770-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 25770-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 25770-1-AP (Drebrin antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules K-562, cellules Neuro-2a, cellules SH-SY5Y, tissu cérébral de souris
• Résultats positifs en IF: cellules MCF-7, cellules HepG2, cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

25770-1-AP cible Drebrin dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Drebrin Protéine recombinante Ag22634
• Nom complet: drebrin 1
• Poids moléculaire observé: 100-130 kDa
• Numéro d’acquisition GenBank: BC007567
• Symbole du gène: DBN1
• Identification du gène (NCBI): 1627
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Drebrin (DBN1) is an actin binding and stabilizing protein with roles in endocytosis, formation of dendrite spines in neurons and coordinating cell-cell synapses in immune cells. Preferentially expressed in the brain, it is highly localized in dendritic spines and regulates spine shapes. It has two isoforms that are splice variants, with drebrin A being expressed in neural cells, and drebrin E is present in several other cell types. Recently it has been reported that circulating DBN1 levels correlated with retinal nerve fiber layer defect and may reflect the severity of retinal ganglion cells injury in glaucoma patients.

Publications

Species	Application	Title
human	IF	Int J Mol Sci; Drebrin Regulates Acetylcholine Receptor Clustering and Organization of Microtubules at the Postsynaptic Machinery; Authors - Paloma Alvarez-Suarez