Anticorps Monoclonal anti-Drebrin

Drebrin Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

IF/ICC, IF-P, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

1A12H1

N° de cat : CL594-67589

Synonymes

DBN1, drebrin 1, Developmentally-regulated brain protein, DBN 1, 1A12H1

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Galerie de données de validation

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  IF Staining of mouse brain using CL594-67589

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 Drebrin antibody (CL594-67589, Clone: 1A12H1 ) at dilution of 1:200.

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  IF Staining of SH-SY5Y using CL594-67589

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite®594 Drebrin antibody (CL594-67589, Clone: 1A12H1 ) at dilution of 1:200.

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  FC experiment of SH-SY5Y using CL594-67589

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Drebrin (CL594-67589, Clone:1A12H1) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF-P: tissu cérébral de souris,
• Résultats positifs en IF/ICC: cellules SH-SY5Y,
• Résultats positifs en FC (Intra): cellules SH-SY5Y,

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-67589 cible Drebrin dans les applications de IF/ICC, IF-P, FC (Intra) et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Drebrin Protéine recombinante Ag22635
• Nom complet: drebrin 1
• Poids moléculaire observé: 110-120 kDa
• Numéro d’acquisition GenBank: BC007567
• Symbole du gène: Drebrin
• Identification du gène (NCBI): 1627
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Drebrin (DBN1) is an actin binding and stabilizing protein with roles in endocytosis, formation of dendrite spines in neurons and coordinating cell-cell synapses in immune cells. Preferentially expressed in the brain, it is highly localized in dendritic spines and regulates spine shapes. It has two isoforms that are splice variants, with drebrin A being expressed in neural cells, and drebrin E is present in several other cell types. Recently it has been reported that circulating DBN1 levels correlated with retinal nerve fiber layer defect and may reflect the severity of retinal ganglion cells injury in glaucoma patients.

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