Anticorps Recombinant de lapin anti-E-cadherin

E-cadherin Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241282A8

N° de cat : 84111-2-PBS

Synonymes

241282A8, cadherin 1, Cdh1, Ecad, L CAM

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Galerie de données de validation

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  WB analysis using 84111-2-RR (same clone as 84111-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84111-2-RR (E-Cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84111-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP01047-2

  The mean E-Cadherin concentration was determined to be 10.6 ng/mL in mouse liver tissue extract based on a 5.0 mg/mL extract load.

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  Sandwich ELISA sample test of MP01047-2

  Serum of six mice was measured. The E-Cadherin concentration of detected samples was determined to be 189.4 ng/mL with a range of 166.7-209.1 ng/mL.

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  Sandwich ELISA standard curve of MP01047-2

  Sandwich ELISA standard curve of MP01047-2, Mouse E-Cadherin Recombinant Matched Antibody Pair - PBS only. 84111-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0975. 84111-2-PBS was HRP conjugated as the detection antibody. Range: 9.77-625 pg/mL.

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  IF Staining of A431 using 84111-2-RR (same clone as 84111-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using E-Cadherin antibody (84111-2-RR, Clone: 241282A8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84111-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01047-2

  Cytometric bead array standard curve of MP01047-2, MOUSE E-Cadherin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84111-1-PBS. Detection antibody: 84111-2-PBS. Standard: Eg0975. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 84111-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 84111-2-PBS against Mouse E-cadherin was performed. The affinity constant is 0.808 nM.

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  Affinity and Kinetic Characterization of 84111-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84111-2-RR against Mouse E-Cadherin were performed. The affinity constant is below 1 pM.

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Informations sur le produit

84111-2-PBS cible E-cadherin dans les applications de WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: E-cadherin Protéine recombinante Eg0975
• Nom complet: cadherin 1
• Masse moléculaire calculée: 98kDa
• Poids moléculaire observé: 135 kDa
• Numéro d’acquisition GenBank: NM_009864.3
• Symbole du gène: E-cadherin
• Identification du gène (NCBI): 12550
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness. E-cadherin is sensitive to trypsin digestion in the absence of Ca2+.

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