Anticorps Recombinant de lapin anti-E-cadherin

E-cadherin Recombinant Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

242018D3

N° de cat : 84646-3-RR

Synonymes

242018D3, Cadherin-1, CD324, Cdh1, E-Cad/CTF1

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Galerie de données de validation

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  WB analysis using 84646-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84646-3-RR (E-Cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat colon using 84646-3-RR

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 84646-3-RR (E-Cadherin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat colon using 84646-3-RR

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 84646-3-RR (E-Cadherin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat colon using 84646-3-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat colon tissue using E-Cadherin antibody (84646-3-RR, Clone: 242018D3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse colon using 84646-3-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using E-Cadherin antibody (84646-3-RR, Clone: 242018D3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84646-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84646-3-RR against Rat E-Cadherin were performed. The affinity constant is 1.84 nM.

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Applications testées

• Résultats positifs en WB: cellules Caco-2, cellules A431
• Résultats positifs en IHC: tissu de côlon de rat, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de côlon de souris, tissu de côlon de rat

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84646-3-RR cible E-cadherin dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: E-cadherin Protéine recombinante Eg1229
• Nom complet: cadherin 1
• Masse moléculaire calculée: 99 kDa
• Poids moléculaire observé: 125 kDa
• Numéro d’acquisition GenBank: NM_031334.1
• Symbole du gène: Cdh1
• Identification du gène (NCBI): 83502
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.

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