Anticorps Recombinant de lapin anti-E-cadherin

E-cadherin Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

241282C1

N° de cat : 98140-1-PBS

Synonymes

241282C1, cadherin 1, Cdh1, Ecad, L CAM

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Galerie de données de validation

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  FC experiment of MDCK using 98140-1-RR (same clone as 98140-1-PBS)

  1x10^6 MDCK cells were surface stained with 0.25 ug Anti-Mouse CD324 (E-cadherin) Rabbit Recombinant Antibody (98140-1-RR, Clone:241282C1) (red) or Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98140-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98140-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98140-1-RR against Mouse CD324 (E-cadherin) were performed. The affinity constant is 3.73 nM.

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Informations sur le produit

98140-1-PBS cible E-cadherin dans les applications de FC et montre une réactivité avec des échantillons canin, souris

• Réactivité: canin, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: E-cadherin Protéine recombinante Eg0975
• Nom complet: cadherin 1
• Masse moléculaire calculée: 98kDa
• Numéro d’acquisition GenBank: NM_009864.3
• Symbole du gène: E-cadherin
• Identification du gène (NCBI): 12550
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CD324, CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.

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