Anticorps Monoclonal anti-E-cadherin

E-cadherin Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

3F6F3

N° de cat : 60902-1-Ig

Synonymes

Arc 1, Cadherin 1, Cadherin-1, CAM 120/80, CD324

---

Galerie de données de validation

• 

  WB analysis using 60902-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60902-1-Ig (E-cadherin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60902-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60902-1-Ig (E-cadherin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HaCaT using 60902-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HaCaT cells using E-cadherin antibody (60902-1-Ig, Clone: 3F6F3 ) at dilution of 1:4000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

---

Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A431, cellules LNCaP, cellules MCF-7
• Résultats positifs en IF/ICC: cellules HaCaT,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

60902-1-Ig cible E-cadherin dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: E-cadherin Protéine recombinante Eg1112
• Nom complet: cadherin 1, type 1, E-cadherin (epithelial)
• Masse moléculaire calculée: 97 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: NM_004360.5
• Symbole du gène: E-cadherin
• Identification du gène (NCBI): 999
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness. E-cadherin is sensitive to trypsin digestion in the absence of Ca2+.

{{ptg:RelatedPrimaryAntibodies}}