Anticorps Monoclonal anti-E-cadherin

E-cadherin Monoclonal Antibody for IF-P, FC

Hôte / Isotype

Rat / IgG1, kappa

Réactivité testée

canin, Humain, souris

Applications

IF-P, FC

Conjugaison

Non conjugué

CloneNo.

DECMA-1

N° de cat : 65241-1-Ig

Synonymes

CD324 (E-cadherin), cadherin 1, Cdh1, Ecad, L CAM

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Galerie de données de validation

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  IF Staining of mouse colon using 65241-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CD324 (E-cadherin) antibody (65241-1-Ig, Clone: DECMA-1 ) at dilution of 1:1250 and Rhodamine (TRITC)-conjugated Goat Anti-Rat IgG(H+L).

• 

  IF Staining of mouse colon using 65241-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CD324 (E-cadherin) antibody (65241-1-Ig, Clone: DECMA-1 ) at dilution of 1:1250 and Rhodamine (TRITC)-conjugated Goat Anti-Rat IgG(H+L).

• 

  IF Staining of mouse colon using 65241-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using CD324 (E-cadherin) antibody (65241-1-Ig, Clone: DECMA-1 ) at dilution of 1:100 and Fluorescein (FITC)-conjugated Goat Anti-Rat IgG(H+L) SA00003-11. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of MDCK using 65241-1-Ig

  1X10^6 MDCK cells were surface stained with 0.2 ug Anti-Mouse CD324 (E-cadherin) (65241-1-Ig, Clone:DECMA-1) and APC-conjugated Goat Anti-Rat IgG at dilution 1:1000. Cells were not fixed.

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Applications testées

• Résultats positifs en IF-P: tissu de côlon de souris,
• Résultats positifs en cytométrie: cellules MDCK,

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:625-1:2500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

65241-1-Ig cible E-cadherin dans les applications de IF-P, FC et montre une réactivité avec des échantillons canin, Humain, souris

• Réactivité: canin, Humain, souris
• Hôte / Isotype: Rat / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Mouse embryonal carcinoma cell line PCC4 Aza RI
• Nom complet: cadherin 1
• Numéro d’acquisition GenBank: BC098501
• Symbole du gène: E-cadherin
• Identification du gène (NCBI): 12550
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. It has been regarded as a marker for spermatogonial stem cells in mice(PMID:23509752). E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.

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