Anticorps Recombinant de lapin anti-E-cadherin

E-cadherin Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(6)

CloneNo.

240159C10

N° de cat : 80541-5-RR

Synonymes

CD324, CAM 120/80, Cadherin-1, Cadherin 1, Arc 1

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Galerie de données de validation

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  WB analysis using 80541-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80541-5-RR (E-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 80541-5-RR

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 80541-5-RR (E-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 80541-5-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 80541-5-RR (E-cadherin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 80541-5-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using E-cadherin antibody (80541-5-RR, Clone: 240159C10 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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  Affinity and Kinetic Characterization of 80541-5-RR

  Biolayer interferometry (BLl) kinetic assays of 80541-5-RR against Human E-cadherin were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HCT 116, cellules A431, cellules Caco-2, cellules MCF-7, cellules T-47D
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IF: See 1 publications below

Informations sur le produit

80541-5-RR cible E-cadherin dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: E-cadherin Protéine recombinante Ag14973
• Nom complet: cadherin 1, type 1, E-cadherin (epithelial)
• Masse moléculaire calculée: 882 aa, 97 kDa
• Poids moléculaire observé: 135 kDa
• Numéro d’acquisition GenBank: BC141838
• Symbole du gène: E-cadherin
• Identification du gène (NCBI): 999
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.

Publications

Species	Application	Title
human	WB	Sci Rep; Hypoxia and lipid metabolism related genes drive proliferation migration and immune infiltration mechanisms in colorectal cancer subtyping; Authors - Shansong Huang
human	WB	J Cancer Res Clin Oncol; CircWDR78 inhibits the development of colorectal cancer by regulating the miR-653-3p/RGS4 axis; Authors - Chu Hao
mouse	WB,IF	Sci Rep; Molecular mechanisms of lung injury from ultra high and conventional dose rate pulsed radiation based on 4D DIA proteomics study; Authors - MengHua Li
human	WB	Int Immunopharmacol; Role of COL5A1 in lung squamous cell Carcinoma: Prognostic Implications and therapeutic potential; Authors - Chengjuan Zhang
human	WB	Pharmaceuticals (Basel); Antitumor Activity of USP7 Inhibitor GNE-6776 in Non-Small Cell Lung Cancer Involves Regulation of Epithelial-Mesenchymal Transition, Cell Cycle, Wnt/β-Catenin, and PI3K/AKT/mTOR Pathways; Authors - Lipeng Wu
human	WB	Nat Commun; Splicing diversity enhances the molecular classification of pituitary neuroendocrine tumors; Authors - Yue Huang

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