Anticorps Recombinant de lapin anti-E-cadherin

E-cadherin Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241098G6

N° de cat : 83991-4-PBS

Synonymes

241098G6, Arc 1, Cadherin 1, Cadherin-1, CAM 120/80

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Galerie de données de validation

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  WB analysis using 83991-4-RR (same clone as 83991-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83991-4-RR (E-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

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  WB analysis using 83991-4-RR (same clone as 83991-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83991-4-RR (E-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 83991-4-RR (same clone as 83991-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 83991-4-RR (same clone as 83991-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83991-4-RR (same clone as 83991-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83991-4-RR (same clone as 83991-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 83991-4-RR (same clone as 83991-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 83991-4-RR (same clone as 83991-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using E-cadherin antibody (83991-4-RR, Clone: 241098G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 83991-4-RR (same clone as 83991-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using E-cadherin antibody (83991-4-RR, Clone: 241098G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83991-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83991-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83991-4-RR against Human E-cadherin were performed. The affinity constant is 2.78 nM.

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Informations sur le produit

83991-4-PBS cible E-cadherin dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: E-cadherin Protéine recombinante Eg1112
• Nom complet: cadherin 1, type 1, E-cadherin (epithelial)
• Masse moléculaire calculée: 97 kDa
• Poids moléculaire observé: 135 kDa
• Numéro d’acquisition GenBank: NM_004360.5
• Symbole du gène: E-cadherin
• Identification du gène (NCBI): 999
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.

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