Anticorps Recombinant de lapin anti-E-cadherin

E-cadherin Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

241098G6

N° de cat : 83991-4-RR

Synonymes

CD324, CAM 120/80, Cadherin-1, Cadherin 1, Arc 1

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Galerie de données de validation

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  WB analysis using 83991-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83991-4-RR (E-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 83991-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83991-4-RR (E-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 83991-4-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 83991-4-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 83991-4-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 83991-4-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 83991-4-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83991-4-RR (E-cadherin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 83991-4-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using E-cadherin antibody (83991-4-RR, Clone: 241098G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of MCF-7 using 83991-4-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using E-cadherin antibody (83991-4-RR, Clone: 241098G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83991-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83991-4-RR against Human E-cadherin were performed. The affinity constant is 2.78 nM.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HCT 116, cellules HepG2, cellules T-47D
• Résultats positifs en IHC: tissu de cancer du côlon humain, human ovary cancer tissue; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83991-4-RR cible E-cadherin dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: cadherin 1, type 1, E-cadherin (epithelial)
• Masse moléculaire calculée: 97 kDa
• Poids moléculaire observé: 135 kDa
• Numéro d’acquisition GenBank: NM_004360.5
• Symbole du gène: E-cadherin
• Identification du gène (NCBI): 999
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.

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