Anticorps Recombinant de lapin anti-CD324 (E-cadherin)

CD324 (E-cadherin) Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

240159B4

N° de cat : 98030-2-RR

Synonymes

E-cadherin, CD324, CAM 120/80, Cadherin-1, Cadherin 1

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Galerie de données de validation

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  FC experiment of MCF-7 using 98030-2-RR

  1x10^6 MCF-7 cells were surface stained with 0.25 ug Anti-Human CD324 (E-cadherin) Rabbit Recombinant Antibody (98030-2-RR, Clone:240159B4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells should be stained prior to fixation.

• 

  FC experiment of HT-29 using 98030-2-RR

  1x10^6 HT-29 cells were surface stained with 0.25 ug Anti-Human E-cadherin Rabbit Recombinant Antibody (98030-2-RR, Clone:240159B4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells should be stained prior to fixation.

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  Affinity and Kinetic Characterization of 98030-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98030-2-RR against Human E-cadherin were performed. The affinity constant is 0.204 nM.

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Applications testées

• Résultats positifs en cytométrie: cellules MCF-7, cellules HT-29

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98030-2-RR cible CD324 (E-cadherin) dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD324 (E-cadherin) Protéine recombinante Ag14973
• Nom complet: cadherin 1, type 1, E-cadherin (epithelial)
• Masse moléculaire calculée: 882 aa, 97 kDa
• Numéro d’acquisition GenBank: BC141838
• Symbole du gène: E-cadherin
• Identification du gène (NCBI): 999
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CD324, CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.

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