Anticorps Recombinant de lapin anti-E-cadherin

E-cadherin Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat

Applications

FC

Conjugaison

Biotin

CloneNo.

242018A7

N° de cat : Biotin-FcA98323

Synonymes

Cadherin-1, CD324, Cdh1, E-Cad/CTF1, E-Cad/CTF2

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Galerie de données de validation

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  FC experiment of PC-12 using Biotin-FcA98323

  1x10^6 PC-12 cells were surface stained with 0.25 ug Biotin Anti-Rat E-cadherin Rabbit RecAb (Biotin-FcA98323, Clone:242018A7) (red) or 0.25 ug Biotin-conjugated IgG control Rabbit PolyAb (Biotin-FcA98136) (blue), and PE-conjugated streptavidin. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: cellules PC-12,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

Biotin-FcA98323 cible E-cadherin dans les applications de FC et montre une réactivité avec des échantillons rat

• Réactivité: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: E-cadherin Protéine recombinante Eg1229
• Nom complet: cadherin 1
• Masse moléculaire calculée: 99 kDa
• Numéro d’acquisition GenBank: NM_031334.1
• Symbole du gène: Cdh1
• Identification du gène (NCBI): 83502
• Conjugaison: Biotin
• Excitation/Emission maxima wavelengths: -
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CD324, CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. It has been regarded as a marker for spermatogonial stem cells in mice(PMID:23509752). E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.

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