• Phare
  • Validé par KD/KO

Anticorps Monoclonal anti-E-cadherin

E-cadherin Monoclonal Antibody for IF

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc

Applications

IF

Conjugaison

CoraLite®555 Fluorescent Dye

CloneNo.

6B11F11

N° de cat : CL555-60335

Synonymes

Arc 1, Cadherin 1, CAM 120/80, CD324, CDH1, CDHE, E cadherin, ECAD, E-cadherin, Epithelial cadherin, LCAM, UVO, Uvomorulin



Applications testées

Résultats positifs en IFcellules MCF-7,

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL555-60335 cible E-cadherin dans les applications de IF et montre une réactivité avec des échantillons Humain, porc

Réactivité Humain, porc
Hôte / Isotype Mouse / IgG2b
Clonalité Monoclonal
Type Anticorps
Immunogène E-cadherin Protéine recombinante Ag15085
Nom complet cadherin 1, type 1, E-cadherin (epithelial)
Masse moléculaire calculée 882 aa, 97 kDa
Poids moléculaire observé 120 kDa
Numéro d’acquisition GenBankBC141838
Symbole du gène CDH1
Identification du gène (NCBI) 999
Conjugaison CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. It has been regarded as a marker for spermatogonial stem cells in mice(PMID:23509752). E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.

Protocole

Product Specific Protocols
IF protocol for CL555 E-cadherin antibody CL555-60335Download protocol
Standard Protocols
Click here to view our Standard Protocols