Anticorps Polyclonal de lapin anti-E-selectin/CD62E

E-selectin/CD62E Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20894-1-PBS

Synonymes

CD62E, E-selectin, CD62 antigen-like family member E, ELAM1, ELAM 1

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Galerie de données de validation

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  WB analysis using 20894-1-AP (same clone as 20894-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20894-1-AP (E-selectin/CD62E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20894-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 20894-1-AP (same clone as 20894-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 20894-1-AP (E-selectin /CD62E antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20894-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 20894-1-AP (same clone as 20894-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 20894-1-AP (E-selectin /CD62E antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20894-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 20894-1-AP (same clone as 20894-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20894-1-AP (E-selectin / CD62E antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20894-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 20894-1-AP (same clone as 20894-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20894-1-AP (E-selectin / CD62E antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20894-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20894-1-PBS cible E-selectin/CD62E dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: E-selectin/CD62E Protéine recombinante Ag14944
• Nom complet: selectin E
• Masse moléculaire calculée: 610 aa, 67 kDa
• Poids moléculaire observé: 115 kDa
• Numéro d’acquisition GenBank: BC142711
• Symbole du gène: CD62E
• Identification du gène (NCBI): 6401
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

E-selectin, also known as ELAM-1 or CD62E, is a member of the selectin family of adhesion molecules that also includes L-selectin and P-selectin. E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS). Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. (PMID: 2827173; 1382417; 8943958)

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