Anticorps Recombinant de lapin anti-E-selectin/CD62E

E-selectin/CD62E Recombinant Antibody for FC, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

241767H6

N° de cat : 98189-1-PBS

Synonymes

CD62E, E-selectin, 241767H6, CD62 antigen-like family member E, E selectin

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Galerie de données de validation

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  Sandwich ELISA standard curve of MP01636-3

  Sandwich ELISA standard curve of MP01636-3, Human E-selectin / CD62E Recombinant Matched Antibody Pair - PBS only. 84887-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02341. 98189-1-PBS was HRP conjugated as the detection antibody. Range: 9.77-625 pg/mL

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  Sample test of MP01636-3

  Serum of eight individual healthy human donors was measured. The human E-selectin / CD62E concentration of detected samples was determined to be 12.99 ng/mL with a range of 4.01 - 24.83 ng/mL.

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  FC experiment of HUVEC using 98189-1-RR (same clone as 98189-1-PBS)

  1x10^6 untreated or TNF alpha treated HUVEC cells were surface stained with 0.25 ug Anti-Human E-selectin/CD62E Rabbit Recombinant Antibody (98189-1-RR, Clone: 241767H6) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98189-1-PBS in a different storage buffer formulation.

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  FC experiment of HUVEC using 98189-1-RR (same clone as 98189-1-PBS)

  1x10^6 TNF alpha treated HUVEC cells were surface stained with 0.25 ug Anti-Human E-selectin/CD62E Rabbit Recombinant Antibody (98189-1-RR, Clone: 241767H6) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated HUVEC cells were surface stained with 0.25 ug Anti-Human E-selectin/CD62E Rabbit Recombinant Antibody (98189-1-RR, Clone: 241767H6) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were not fixed. This data was developed using the same antibody clone with 98189-1-PBS in a different storage buffer formulation.

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Informations sur le produit

98189-1-PBS cible E-selectin/CD62E dans les applications de FC, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: selectin E
• Masse moléculaire calculée: 610 aa, 67 kDa
• Numéro d’acquisition GenBank: BC142711
• Symbole du gène: CD62E
• Identification du gène (NCBI): 6401
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

E-selectin, also known as CD62E or ELAM-1, is a member of the selectin family of adhesion molecules that also includes L-selectin and P-selectin. E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS). Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. (PMID: 2827173; 1382417; 8943958)

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