Anticorps Recombinant de lapin anti-E-selectin/CD62E

E-selectin/CD62E Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

FITC Plus Fluorescent Dye

CloneNo.

241767H6

N° de cat : FITC-98189

Synonymes

CD62E, E-selectin, 241767H6, CD62 antigen-like family member E, E selectin

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Galerie de données de validation

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  FC experiment of HUVEC using FITC-98189

  1x10^6 untreated or TNF alpha-treated HUVEC cells were surface stained with 5 ul FITC Plus Anti-Human E-selectin/CD62E Rabbit RecAb (FITC-98189, Clone: 241767H6). Cells were not fixed.

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  FC experiment of HUVEC using FITC-98189

  1x10^6 TNF alpha-treated HUVEC cells were surface stained with 5 ul FITC Plus Anti-Human E-selectin/CD62E Rabbit RecAb (FITC-98189, Clone: 241767H6) (red) or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9) (blue). 1x10^6 untreated HUVEC cells were surface stained with 5 ul FITC Plus Anti-Human E-selectin/CD62E Rabbit RecAb (FITC-98189, Clone: 241767H6) (black, dashed). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: TNF alpha treated HUVEC cells

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

FITC-98189 cible E-selectin/CD62E dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: selectin E
• Masse moléculaire calculée: 610 aa, 67 kDa
• Numéro d’acquisition GenBank: BC142711
• Symbole du gène: CD62E
• Identification du gène (NCBI): 6401
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

E-selectin, also known as CD62E or ELAM-1, is a member of the selectin family of adhesion molecules that also includes L-selectin and P-selectin. E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS). Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. (PMID: 2827173; 1382417; 8943958)

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