Anticorps Monoclonal anti-E2F1

• Phare
• Validé par KD/KO

E2F1 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5D7G8

N° de cat : 66515-1-PBS

Synonymes

5D7G8, E2F 1, E2F-1, pRB-binding protein E2F-1, RBAP-1

---

Galerie de données de validation

• 

  WB analysis using 66515-1-Ig (same clone as 66515-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66515-1-Ig (E2F1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66515-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66515-1-Ig (same clone as 66515-1-PBS)

  WB result of E2F1 antibody (66515-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-E2F1 transfected HepG2 cells. This data was developed using the same antibody clone with 66515-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66515-1-PBS cible E2F1 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: E2F1 Protéine recombinante Ag17363
• Nom complet: E2F transcription factor 1
• Masse moléculaire calculée: 437 aa, 47 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC050369
• Symbole du gène: E2F1
• Identification du gène (NCBI): 1869
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Transcription factor E2F1 (E2F1), also known as RBBP3, is a transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. The calculated molecular weight of E2F1 is 47 kDa, but the sumoylated E2F1 is bout 55-60 kDa.

{{ptg:RelatedPrimaryAntibodies}}