Anticorps Recombinant de lapin anti-E2F8

E2F8 Recombinant Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

CloneNo.

251510C2

N° de cat : 86645-1-RR

Synonymes

E2F 8, E2F transcription factor 8, E2F-8, Transcription factor E2F8

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Galerie de données de validation

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  WB analysis using 86645-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86645-1-RR (E2F8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 86645-1-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 86645-1-RR (E2F8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 86645-1-RR

  IP result of anti-E2F8 (IP:86645-1-RR, 4ug; Detection:86645-1-RR 1:600) with HeLa cells lysate 1120 ug.

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  Affinity and Kinetic Characterization of 86645-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86645-1-RR against Human E2F8 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293T, cellules Jurkat
• Résultats positifs en IP: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86645-1-RR cible E2F8 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: E2F8 Protéine recombinante Ag4216
• Nom complet: E2F transcription factor 8
• Masse moléculaire calculée: 867 aa, 94 kDa
• Poids moléculaire observé: 105 kDa
• Numéro d’acquisition GenBank: BC028244
• Symbole du gène: E2F8
• Identification du gène (NCBI): 79733
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

E2F8 is one E2F transcription factor that is essential for orchestrating expression of genes required for cell cycle progression, proliferation, apoptosis and differentiation. E2F8 shows a high degree of resemblance to E2F7 and shares the unique structure of E2F7 by having two distinct domains exhibiting a high degree of similarity to the DNA-binding domain of the E2F familyTogether with E2F7, they possess two DNA-binding domains that are predicted to interact with each other. E2F8 binds consensus E2F sites in a DP-independent manner and represses transcription of E2F-regulated promoters. Ectopic expression of E2F8 inhibits cellular proliferation. The calcualted molecular weight of E2F8 is 94 kDa, but modified E2F8 is about 105 kDa. (PMID: 15897886)

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