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Anticorps Polyclonal de lapin anti-EAAT4

EAAT4 Polyclonal Antibody for WB, IF-Fro, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF-Fro, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12876-1-PBS

Synonymes

SLC1A6, Sodium-dependent glutamate/aspartate transporter

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of A375 cells using EAAT4 Polyclonal antibody (12876-1-AP)

    WB analysis of A375 using 12876-1-AP (same clone as 12876-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 12876-1-AP (EAAT4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12876-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using EAAT4 Polyclonal antibody (12876-1-AP)

    IF Staining of mouse cerebellum using 12876-1-AP (same clone as 12876-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse cerebellum tissue using EAAT4 antibody (12876-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12876-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using EAAT4 Polyclonal antibody (12876-1-AP)

    IF Staining of mouse cerebellum using 12876-1-AP (same clone as 12876-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse cerebellum tissue using EAAT4 antibody (12876-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12876-1-PBS in a different storage buffer formulation.

Informations sur le produit

12876-1-PBS cible EAAT4 dans les applications de WB, IF-Fro, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène EAAT4 Protéine recombinante Ag3554
Nom complet solute carrier family 1 (high affinity aspartate/glutamate transporter), member 6
Masse moléculaire calculée 61.6 kDa
Poids moléculaire observé 60-70 kDa
Numéro d’acquisition GenBankBC028721
Symbole du gène EAAT4
Identification du gène (NCBI) 6511
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Excitatory amino acid transporter 4 (EAAT4) is a neuronal glutamate transporter responsible for the reuptake of synaptically released glutamate. The EAAT4 protein is highly enriched in Purkinje cells of the cerebellum, although it is not restricted to these cells (18770868). While the predicted molecular weight of EAAT4 is 62 kDa, considerable heterogeneity in transporter size has been reported in the brain with molecular weights ranging from 50 to 120 kDa as a result of differential glycosylation and/or phosphorylation (24056007). This antibody recognizes 60-70 kDa of EAAT4 in mouse brain and several other lysate.

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