Anticorps Polyclonal de lapin anti-EAF2

EAF2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11172-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HEK-293 using 11172-1-AP (same clone as 11172-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11172-1-AP (EAF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11172-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 11172-1-AP (same clone as 11172-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11172-1-AP (EAF2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11172-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 11172-1-AP (same clone as 11172-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11172-1-AP (EAF2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11172-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 11172-1-AP (same clone as 11172-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11172-1-AP (EAF2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11172-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 11172-1-AP (same clone as 11172-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 11172-1-AP (EAF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11172-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11172-1-PBS cible EAF2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EAF2 Protéine recombinante Ag1637
• Nom complet: ELL associated factor 2
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC014209
• Symbole du gène: EAF2
• Identification du gène (NCBI): 55840
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ELL associated factor 1 and ELL associated factor 2 (EAF1/2 factors) are reported to play important roles in tumor suppression and embryogenesis. The tumor suppressor EAF2 is regulated by androgen signaling and associated with prostate cancer. Eaf factors have been revealed to modulate mesoderm and neural patterning by inhibiting canonical Wnt signaling, and high activity of canonical Wnt/β-catenin is revealed in eafs morphants (PMID: 23364330). Catalog#11172-1-AP is a rabbit polyclonal antibody raised against the full-length of human EAF2.

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