Anticorps Polyclonal de lapin anti-EBP2

• Phare
• Validé par KD/KO

EBP2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 15973-1-AP

Synonymes

EBNA1 binding protein 2, EBNA1BP2, EBP2, NOBP, Nucleolar protein p40, P40

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Galerie de données de validation

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  WB analysis of MCF-7 using 15973-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 15973-1-AP (EBP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15973-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15973-1-AP (EBP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15973-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15973-1-AP (EBP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 15973-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15973-1-AP (EBP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15973-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15973-1-AP (EBP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 15973-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15973-1-AP (EBP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 15973-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15973-1-AP (EBP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules A549, cellules HEK-293, cellules HeLa, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

15973-1-AP cible EBP2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EBP2 Protéine recombinante Ag8760
• Nom complet: EBNA1 binding protein 2
• Masse moléculaire calculée: 306 aa, 35 kDa
• Poids moléculaire observé: 35-38 kDa
• Numéro d’acquisition GenBank: BC009175
• Symbole du gène: EBNA1BP2
• Identification du gène (NCBI): 10969
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Mol Oncol; EBP2, a novel NPM-ALK-interacting protein in the nucleolus, contributes to the proliferation of ALCL cells by regulating tumor suppressor p53.; Authors - Yuki Uchihara ; KD Validated