Anticorps Polyclonal de lapin anti-ECH1

ECH1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 11385-1-AP

Synonymes

ECH1, HPXEL

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Galerie de données de validation

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  WB analysis of mouse liver using 11385-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11385-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11385-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11385-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 11385-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 11385-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11385-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 11385-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11385-1-AP (ECH1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HepG2 using 11385-1-AP

  Immunofluorescent analysis of HepG2 cells, using ECH1 antibody 11385-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HepG2, cellules Jurkat, cellules K-562, cellules L02, tissu cardiaque de souris
• Résultats positifs en IHC: tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

11385-1-AP cible ECH1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ECH1 Protéine recombinante Ag1931
• Nom complet: enoyl Coenzyme A hydratase 1, peroxisomal
• Masse moléculaire calculée: 328 aa, 36 kDa
• Poids moléculaire observé: 30-36 kDa
• Numéro d’acquisition GenBank: BC017408
• Symbole du gène: ECH1
• Identification du gène (NCBI): 1891
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	J Clin Invest; Ketohexokinase C blockade ameliorates fructose-induced metabolic dysfunction in fructose-sensitive mice.; Authors - Miguel A Lanaspa
mouse	WB	Hepatology; High fat and high sucrose (western) diet induce steatohepatitis that is dependent on fructokinase.; Authors - Ishimoto Takuji T
mouse	WB	Front Cell Dev Biol; Single-Cell Transcriptomics Reveals Splicing Features of Adult Neural Stem Cells in the Subventricular Zone.; Authors - Yanlu Wang
mouse	WB	Eur J Immunol; YY1 control of mitochondrial-related genes does not account for regulation of immunoglobulin class switch recombination in mice.; Authors - Satabdi Nandi