Anticorps Polyclonal de lapin anti-ECH1

ECH1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 11385-1-AP

Synonymes

HPXEL, EC:5.3.3.-, Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial

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Galerie de données de validation

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  WB analysis of mouse liver using 11385-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11385-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 11385-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 11385-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11385-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11385-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11385-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 11385-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11385-1-AP (ECH1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HepG2, cellules Jurkat, cellules K-562, cellules L02, tissu cardiaque de souris
• Résultats positifs en IHC: tissu de côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below

Informations sur le produit

11385-1-AP cible ECH1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ECH1 Protéine recombinante Ag1931
• Nom complet: enoyl Coenzyme A hydratase 1, peroxisomal
• Masse moléculaire calculée: 328 aa, 36 kDa
• Poids moléculaire observé: 30-36 kDa
• Numéro d’acquisition GenBank: BC017408
• Symbole du gène: ECH1
• Identification du gène (NCBI): 1891
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Enoyl coenzyme A hydratase 1 (ECH1), is first recog-nized as a key component in mitochondrial fatty acid β-oxidation, and subsequent studies have demonstrated that it regulates multiple pathophysiological processes (PMID:33813878). The MW of ECH1 is 36 kDa,and can be detected as 30-36 kDa.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity; Authors - Zilong Zhang
mouse	WB	J Clin Invest; Ketohexokinase C blockade ameliorates fructose-induced metabolic dysfunction in fructose-sensitive mice.; Authors - Miguel A Lanaspa
mouse	WB	Hepatology; High fat and high sucrose (western) diet induce steatohepatitis that is dependent on fructokinase.; Authors - Ishimoto Takuji T
mouse	WB	Front Cell Dev Biol; Single-Cell Transcriptomics Reveals Splicing Features of Adult Neural Stem Cells in the Subventricular Zone.; Authors - Yanlu Wang
mouse	WB	Eur J Immunol; YY1 control of mitochondrial-related genes does not account for regulation of immunoglobulin class switch recombination in mice.; Authors - Satabdi Nandi
human	WB	Cell Death Differ; C-IGF1R encoded by cIGF1R acts as a molecular switch to restrict mitophagy of drug-tolerant persister tumour cells in non-small cell lung cancer; Authors - Hui Wang

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