Anticorps Polyclonal de lapin anti-ECH1

ECH1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11385-1-PBS

Synonymes

HPXEL, EC:5.3.3.-, Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial

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Galerie de données de validation

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  WB analysis of mouse liver using 11385-1-AP (same clone as 11385-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11385-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 11385-1-AP (same clone as 11385-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11385-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart using 11385-1-AP (same clone as 11385-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11385-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 11385-1-AP (same clone as 11385-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11385-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 11385-1-AP (same clone as 11385-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11385-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 11385-1-AP (same clone as 11385-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11385-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 11385-1-AP (same clone as 11385-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11385-1-AP (ECH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11385-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 11385-1-AP (same clone as 11385-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11385-1-AP (ECH1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11385-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11385-1-PBS cible ECH1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ECH1 Protéine recombinante Ag1931
• Nom complet: enoyl Coenzyme A hydratase 1, peroxisomal
• Masse moléculaire calculée: 328 aa, 36 kDa
• Poids moléculaire observé: 30-36 kDa
• Numéro d’acquisition GenBank: BC017408
• Symbole du gène: ECH1
• Identification du gène (NCBI): 1891
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Enoyl coenzyme A hydratase 1 (ECH1), is first recog-nized as a key component in mitochondrial fatty acid β-oxidation, and subsequent studies have demonstrated that it regulates multiple pathophysiological processes (PMID:33813878). The MW of ECH1 is 36 kDa,and can be detected as 30-36 kDa.

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