Anticorps Monoclonal anti-ECH1

• Phare
• Validé par KD/KO

ECH1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C9G5

N° de cat : 68139-1-PBS

Synonymes

1C9G5, Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial, EC:5.3.3.-, HPXEL

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Galerie de données de validation

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  WB analysis using 68139-1-Ig (same clone as 68139-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68139-1-Ig (ECH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68139-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 68139-1-Ig (same clone as 68139-1-PBS)

  WB result of ECH1 antibody (68139-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ECH1 transfected HepG2 cells. This data was developed using the same antibody clone with 68139-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 68139-1-Ig (same clone as 68139-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68139-1-Ig (ECH1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68139-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 68139-1-Ig (same clone as 68139-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68139-1-Ig (ECH1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68139-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68139-1-Ig (same clone as 68139-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ECH1 antibody (68139-1-Ig, Clone: 1C9G5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68139-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68139-1-PBS cible ECH1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ECH1 Protéine recombinante Ag32661
• Nom complet: enoyl Coenzyme A hydratase 1, peroxisomal
• Masse moléculaire calculée: 328 aa, 36 kDa
• Poids moléculaire observé: 33-36 kDa
• Numéro d’acquisition GenBank: BC017408
• Symbole du gène: ECH1
• Identification du gène (NCBI): 1891
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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