Anticorps Monoclonal anti-ECHS1

• Phare
• Validé par KD/KO

ECHS1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

CloneNo.

2B9D5

N° de cat : 66117-1-Ig

Synonymes

mECH, Enoyl-CoA hydratase, mitochondrial, Enoyl-CoA hydratase 1, ECHM, EC:4.2.1.17

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Galerie de données de validation

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  WB analysis of HepG2 using 66117-1-Ig

  WB result of ECHS1 antibody (66117-1-Ig, 1:20,000) with si-Control and si-ECHS1 transfected HepG2 cells.

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  WB analysis of MCF-7 using 66117-1-Ig

  WB result of ECHS1 antibody (66117-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ECHS1 transfected MCF-7 cells.

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  WB analysis using 66117-1-Ig

  Various cells were subjected to SDS PAGE followed by western blot with 66117-1-Ig (ECHS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 66117-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66117-1-Ig (ECHS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 66117-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66117-1-Ig (ECHS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 66117-1-Ig

  IP result of anti-ECHS1 (IP:66117-1-Ig, 3ug; Detection:66117-1-Ig 1:1000) with HepG2 cells lysate 3600ug.

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  IHC staining of human prostate cancer using 66117-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66117-1-Ig (ECHS1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 66117-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66117-1-Ig (ECHS1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 66117-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66117-1-Ig (ECHS1 Antibody) at dilution of 1:50.

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  IHC staining of human prostate cancer using 66117-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66117-1-Ig (ECHS1 Antibody) at dilution of 1:50.

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  IHC staining of human liver using 66117-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66117-1-Ig (ECHS1 Antibody) at dilution of 1:50.

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  IHC staining of human liver using 66117-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66117-1-Ig (ECHS1 Antibody) at dilution of 1:50.

• 

  IF Staining of HeLa using 66117-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ECHS1 antibody (66117-1-Ig, Clone: 2B9D5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules COLO 320, cellules HEK-293, cellules HepG2, cellules L02, cellules MCF-7, cellules PC-3, cellules T47D, tissu testiculaire humain
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:20000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

66117-1-Ig cible ECHS1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ECHS1 Protéine recombinante Ag16775
• Nom complet: enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC008906
• Symbole du gène: ECHS1
• Identification du gène (NCBI): 1892
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Enoyl-coenzyme A hydratase (ECHS1) is a mitochondrial protein which catalyzes the hydration of 2-trans-enoyl-coenzyme A intermediates to L-3-hydroxyacyl-coenzyme A, playing key role in metabolism of fatty acids in mitochondria. ECHS1 is highly expressed in muscle, liver and fibroblasts. Altered expression of ECHS1 has been considered as a characteristic feature of mitochondria dysfunction. (23275097, 23235493)

Publications

Species	Application	Title
human	WB	J Proteomics; Up-regulation of fatty acid oxidation in the ligament as a contributing factor of ankylosing spondylitis: A comparative proteomic study.; Authors - Wei-Dong Xu
human	WB	Ann Clin Transl Neurol; Deficiency of ECHS1 causes mitochondrial encephalopathy with cardiac involvement.; Authors - Tobias B Haack
human	WB	FEBS Open Bio; The role of SAMM50 in non-alcoholic fatty liver disease: from genetics to mechanisms.; Authors - Zuyin Li
human	WB,IF,IP	Cell Death Dis; ECHS1, an interacting protein of LASP1, induces sphingolipid-metabolism imbalance to promote colorectal cancer progression by regulating ceramide glycosylation.; Authors - Rui Li ; KD Validated
mouse	WB	bioRxiv; Valine and Inflammation Drive Epilepsy in a Mouse Model of ECHS1 Deficiency; Authors - Meghan M Eller ; KO Validated
human	WB	J Cancer; Knockdown of HOXD13 in Oral Squamous Cell Carcinoma Inhibited its Proliferation, Migration, and Influenced Fatty Acid Metabolism; Authors - Xingyue Ma

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