Anticorps Monoclonal anti-ECHS1

• Phare
• Validé par KD/KO

ECHS1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2B9D5

N° de cat : 66117-1-PBS

Synonymes

mECH, Enoyl-CoA hydratase, mitochondrial, Enoyl-CoA hydratase 1, ECHM, EC:4.2.1.17

---

Galerie de données de validation

• 

  WB analysis of HepG2 using 66117-1-Ig (same clone as 66117-1-PBS)

  WB result of ECHS1 antibody (66117-1-Ig, 1:20,000) with si-Control and si-ECHS1 transfected HepG2 cells. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66117-1-Ig (same clone as 66117-1-PBS)

  Various cells were subjected to SDS PAGE followed by western blot with 66117-1-Ig (ECHS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 66117-1-Ig (same clone as 66117-1-PBS)

  WB result of ECHS1 antibody (66117-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ECHS1 transfected MCF-7 cells. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 66117-1-Ig (same clone as 66117-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 66117-1-Ig (ECHS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 66117-1-Ig (same clone as 66117-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66117-1-Ig (ECHS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 66117-1-Ig (same clone as 66117-1-PBS)

  IP result of anti-ECHS1 (IP:66117-1-Ig, 3ug; Detection:66117-1-Ig 1:1000) with HepG2 cells lysate 3600ug. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66117-1-Ig (same clone as 66117-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66117-1-Ig (ECHS1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66117-1-Ig (same clone as 66117-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66117-1-Ig (ECHS1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66117-1-Ig (same clone as 66117-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66117-1-Ig (ECHS1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66117-1-Ig (same clone as 66117-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66117-1-Ig (ECHS1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66117-1-Ig (same clone as 66117-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66117-1-Ig (ECHS1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66117-1-Ig (same clone as 66117-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66117-1-Ig (ECHS1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66117-1-Ig (same clone as 66117-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ECHS1 antibody (66117-1-Ig, Clone: 2B9D5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66117-1-PBS cible ECHS1 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ECHS1 Protéine recombinante Ag16775
• Nom complet: enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC008906
• Symbole du gène: ECHS1
• Identification du gène (NCBI): 1892
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Enoyl-coenzyme A hydratase (ECHS1) is a mitochondrial protein which catalyzes the hydration of 2-trans-enoyl-coenzyme A intermediates to L-3-hydroxyacyl-coenzyme A, playing key role in metabolism of fatty acids in mitochondria. ECHS1 is highly expressed in muscle, liver and fibroblasts. Altered expression of ECHS1 has been considered as a characteristic feature of mitochondria dysfunction. (23275097, 23235493)

{{ptg:RelatedPrimaryAntibodies}}