Anticorps Recombinant de lapin anti-ECHS1

• Phare
• Validé par KD/KO

ECHS1 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5G5

N° de cat : 81611-1-PBS

Synonymes

mECH, Enoyl-CoA hydratase, mitochondrial, Enoyl-CoA hydratase 1, ECHM, EC:4.2.1.17

---

Galerie de données de validation

• 

  WB analysis using 81611-1-RR (same clone as 81611-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81611-1-RR (ECHS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81611-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 81611-1-RR (same clone as 81611-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81611-1-RR (ECHS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81611-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 81611-1-RR (same clone as 81611-1-PBS)

  WB result of ECHS1 antibody (81611-1-RR; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ECHS1 transfected MCF-7 cells. This data was developed using the same antibody clone with 81611-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 81611-1-RR (same clone as 81611-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 81611-1-RR (ECHS1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81611-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 81611-1-RR (same clone as 81611-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 81611-1-RR (ECHS1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81611-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 81611-1-RR (same clone as 81611-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ECHS1 antibody (81611-1-RR, Clone: 5G5 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 81611-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 81611-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81611-1-RR against Human ECHS1 were performed. The affinity constant is 95.5 pM.

---

Informations sur le produit

81611-1-PBS cible ECHS1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ECHS1 Protéine recombinante Ag1842
• Nom complet: enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC008906
• Symbole du gène: ECHS1
• Identification du gène (NCBI): 1892
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Enoyl-coenzyme A hydratase (ECHS1) is a mitochondrial protein which catalyzes the hydration of 2-trans-enoyl-coenzyme A intermediates to L-3-hydroxyacyl-coenzyme A, playing a key role in metabolism of fatty acids in mitochondria. ECHS1 is highly expressed in muscle, liver and fibroblasts. Altered expression of ECHS1 has been considered a characteristic feature of mitochondria dysfunction. (23275097, 23235493)

{{ptg:RelatedPrimaryAntibodies}}