Anticorps Monoclonal anti-ECM1

ECM1 Monoclonal Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

6A12B3

N° de cat : 66023-1-PBS

Synonymes

6A12B3, extracellular matrix protein 1, Secretory component p85

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Galerie de données de validation

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  WB analysis of MCF-7 using 66023-1-Ig (same clone as 66023-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 66023-1-Ig (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66023-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 66023-1-Ig (same clone as 66023-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66023-1-Ig (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66023-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 66023-1-Ig (same clone as 66023-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66023-1-Ig (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66023-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP50074-1

  Sandwich ELISA standard curve of MP50074-1, ECM1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68472-1-PBS. Detection antibody: 66023-1-PBS. Standard: Eg0533. Range: 0.156-10 ng/mL.

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  Sample test of MP50074-1

  Serum of eight individual healthy human donors was measured. The human ECM1 concentration of detected samples was determined to be 254.48 ng/mL with a range of 71.72 - 451.84 ng/mL.

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  IHC staining of human breast cancer using 66023-1-Ig (same clone as 66023-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 66023-1-Ig(ECM1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66023-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66023-1-Ig (same clone as 66023-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 66023-1-Ig(ECM1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66023-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66023-1-Ig (same clone as 66023-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 66023-1-Ig(ECM1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66023-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66023-1-Ig (same clone as 66023-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 66023-1-Ig(ECM1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66023-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66023-1-PBS cible ECM1 dans les applications de WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ECM1 Protéine recombinante Ag17955
• Nom complet: extracellular matrix protein 1
• Masse moléculaire calculée: 540 aa, 61 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC023505
• Symbole du gène: ECM1
• Identification du gène (NCBI): 1893
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Extracellular matrix protein 1 (ECM1) is a glycoprotein involved in a number of biological processes such as such as bone formation, skin differentiation, cell proliferation, and promotes angiostasis. Pathologically, ECM1 contributes to the formation and metastasis of several types of cancer including breast, thyroid and hepatocellular cancers.(21128013) It inhibits MMP9 proteolytic activity.

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