Anticorps Monoclonal anti-ECM1

ECM1 Monoclonal Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

2D1A3

N° de cat : 68472-1-PBS

Synonymes

2D1A3, extracellular matrix protein 1, Secretory component p85

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Galerie de données de validation

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  WB analysis using 68472-1-Ig (same clone as 68472-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68472-1-PBS in a different storage buffer formulation.

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  WB analysis of human plasma using 68472-1-Ig (same clone as 68472-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68472-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 68472-1-Ig (same clone as 68472-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68472-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 68472-1-Ig (same clone as 68472-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68472-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 68472-1-Ig (same clone as 68472-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68472-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP50074-1

  Sandwich ELISA standard curve of MP50074-1, ECM1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68472-1-PBS. Detection antibody: 66023-1-PBS. Standard: Eg0533. Range: 0.156-10 ng/mL.

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  Sample test of MP50074-1

  Serum of eight individual healthy human donors was measured. The human ECM1 concentration of detected samples was determined to be 254.48 ng/mL with a range of 71.72 - 451.84 ng/mL.

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  IHC staining of human skin cancer using 68472-1-Ig (same clone as 68472-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 68472-1-Ig (ECM1 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68472-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68472-1-PBS cible ECM1 dans les applications de WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ECM1 Protéine recombinante Eg0533
• Nom complet: extracellular matrix protein 1
• Masse moléculaire calculée: 540 aa, 61 kDa
• Poids moléculaire observé: 60-80 kDa
• Numéro d’acquisition GenBank: BC023505
• Symbole du gène: ECM1
• Identification du gène (NCBI): 1893
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ECM1 (extracellular matrix protein 1), also known as URBWD. It is located in secreted, extracellular space, and extracellular matrix, which is mainly expressed in esophagus and gall bladder. The gene encodes a soluble protein that is involved in endochondral bone formation, angiogenesis, and tumor biology. It also interacts with a variety of extracellular and structural proteins, contributing to the maintenance of skin integrity and homeostasis. Mutations in this gene are associated with lipoid proteinosis disorder (also known as hyalinosis cutis et mucosae or Urbach-Wiethe disease) that is characterized by generalized thickening of skin, mucosae and certain viscera. The calculated molecular weight of ECM1 is 60 kDa, and this antibody can recognize the 63 kDa isoform of target, moreover, this protein may have glycosylation modification.

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