Anticorps Polyclonal de lapin anti-ECSIT

ECSIT Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 27548-1-PBS

Synonymes

SITPEC, Protein SITPEC, ECSIT homolog (Drosophila)

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Galerie de données de validation

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  WB analysis using 27548-1-AP (same clone as 27548-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27548-1-AP (ECSIT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27548-1-PBS in a different storage buffer formulation.

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  WB analysis using 27548-1-AP (same clone as 27548-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27548-1-AP (ECSIT antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27548-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 27548-1-AP (same clone as 27548-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27548-1-AP (ECSIT antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27548-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 27548-1-AP (same clone as 27548-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27548-1-AP (ECSIT antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27548-1-PBS in a different storage buffer formulation.

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Informations sur le produit

27548-1-PBS cible ECSIT dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ECSIT Protéine recombinante Ag26877
• Nom complet: ECSIT homolog (Drosophila)
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC008279
• Symbole du gène: ECSIT
• Identification du gène (NCBI): 51295
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ECSIT is a cytosolic adaptor protein associated with the toll-like receptor pathway. It has a distinct N-terminal mitochondrial targeting sequence, pentatricopeptide repeat motif, and a C-terminal pleckstrin homology domain. ECSIT regulates many biological processes like embryonic development, inflammation, cardiac function, and assembly of mitochondrial complex I. In macrophages, the activation of TLRs by inflammatory signals promotes the translocation of TRAF6 to mitochondria where it engages ECSIT to stimulate the production of mitochondrial reactive oxygen species (ROS).

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