Anticorps Recombinant de lapin anti-ECSIT

ECSIT Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240223G2

N° de cat : 83295-5-PBS

Synonymes

SITPEC, Protein SITPEC, ECSIT homolog (Drosophila), 240223G2

---

Galerie de données de validation

• 

  WB analysis using 83295-5-RR (same clone as 83295-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83295-5-RR (ECSIT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83295-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 83295-5-RR (same clone as 83295-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83295-5-RR (ECSIT antibody) at dilution of 1:250 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83295-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 83295-5-RR (same clone as 83295-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83295-5-RR (ECSIT antibody) at dilution of 1:250 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83295-5-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 83295-5-RR (same clone as 83295-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ECSIT antibody (83295-5-RR, Clone: 240223G2 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 83295-5-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83295-5-RR (same clone as 83295-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug ECSIT Recombinant antibody (83295-5-RR, Clone:240223G2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83295-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83295-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83295-5-RR against Human ECSIT were performed. The affinity constant is 1.95 nM.

---

Informations sur le produit

83295-5-PBS cible ECSIT dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ECSIT Protéine recombinante Ag26898
• Nom complet: ECSIT homolog (Drosophila)
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC008279
• Symbole du gène: ECSIT
• Identification du gène (NCBI): 51295
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ECSIT is a cytosolic adaptor protein associated with the toll-like receptor pathway. It has a distinct N-terminal mitochondrial targeting sequence, pentatricopeptide repeat motif, and a C-terminal pleckstrin homology domain. ECSIT regulates many biological processes like embryonic development, inflammation, cardiac function, and assembly of mitochondrial complex I. In macrophages, the activation of TLRs by inflammatory signals promotes the translocation of TRAF6 to mitochondria where it engages ECSIT to stimulate the production of mitochondrial reactive oxygen species (ROS).

{{ptg:RelatedPrimaryAntibodies}}