Anticorps Polyclonal de lapin anti-ECT2

ECT2 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

N° de cat : 26557-1-AP

Synonymes

Epithelial cell-transforming sequence 2 oncogene, Protein ECT2

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Galerie de données de validation

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  WB analysis using 26557-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26557-1-AP (ECT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26557-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26557-1-AP (ECT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 26557-1-AP

  IP result of anti-ECT2 (IP:26557-1-AP, 4ug; Detection:26557-1-AP 1:6000) with K-562 cells lysate 1640 ug.

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  IF Staining of A549 using 26557-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using ECT2 antibody (26557-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  FC experiment of A549 using 26557-1-AP

  1x10^6 A549 cells were intracellularly stained with 0.25 ug ECT2 Polyclonal antibody (26557-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293T, cellules K-562
• Résultats positifs en IP: cellules K-562,
• Résultats positifs en IF/ICC: cellules A549,
• Résultats positifs en FC (Intra): cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

26557-1-AP cible ECT2 dans les applications de WB, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ECT2 Protéine recombinante Ag24836
• Nom complet: epithelial cell transforming sequence 2 oncogene
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC112086
• Symbole du gène: ECT2
• Identification du gène (NCBI): 1894
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Epithelial cell transforming sequence 2 (Ect2) is a guanine nucleotide exchange factor (GEF) for the Rho family GTPases, Rac1, RhoA and Cdc42. Ect2 catalyzes the exchange of GDP for GTP, thereby activating the Rho GTPases toward their downstream effectors  (PMID: 35069543). The cellular localization of Ect2 is cell cycle dependent. Ect2 is localized predominantly in the nucleus during interphase due to two functional NLS sequences found within its central hinge region.1 At the onset of mitosis, Ect2 is dispersed throughout the cytoplasm where it associates with the mitotic spindle at metaphase, the cleavage furrow during telophase, and the mid-body during cytokinesis (PMID: 35069543). Besides normal cellular activity, ECT2 also participates in malignant transformation, tumor initiation, and metastasis (PMID: 36572949).

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