Anticorps Polyclonal de lapin anti-EDAR

EDAR Polyclonal Antibody for FC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 18032-1-AP

Synonymes

DL, Downless homolog, Ectodermal dysplasia receptor, ectodysplasin A receptor, ED1R, ED3, ED5, EDA A1 receptor, EDA A1R, EDA1R, EDA3, EDAR, HRM1

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Galerie de données de validation

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  WB analysis of A549 using 18032-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18032-1-AP (EDAR antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis using 18032-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18032-1-AP (EDAR antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 18032-1-AP

  IP Result of anti-EDAR (IP:18032-1-AP, 4ug; Detection:18032-1-AP 1:500) with A549 cells lysate 2800ug.

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  FC experiment of HepG2 using 18032-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug EDAR antibody (18032-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules A431, cellules HEK-293, cellules MCF-7
• Résultats positifs en IP: cellules A549
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

18032-1-AP cible EDAR dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EDAR Protéine recombinante Ag12601
• Nom complet: ectodysplasin A receptor
• Masse moléculaire calculée: 448 aa, 49 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC093872
• Symbole du gène: EDAR
• Identification du gène (NCBI): 10913
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EDAR, also named as DL, is a receptor for EDA isoform A1, but not for EDA isoform A2. It mediates the activation of NF-kappa-B and JNK. EDAR may promote caspase-independent cell death. It is expressed in adjacent or partially overlapping layers in the developing human skin with MW 48 kDa.

Publications

Species	Application	Title
mouse	IF	Development; Involvement of Wnt, Eda and Shh at defined stages of sweat gland development.; Authors - Chang-Yi Cui
mouse	WB	J Pharm Pharmacol; Minimally modified low-density lipoprotein upregulates the ETB and α1 receptors in mouse mesenteric arteries in vivo by activating the PI3K/Akt pathway.; Authors - Zhong-San Zeng
mouse	IF,IHC	J Histochem Cytochem; Spatial and Temporal Expression of Ectodysplasin-A Signaling Pathway Members During Mandibular Condylar Development in Postnatal Mice; Authors - Ludan Xing