Anticorps Polyclonal de lapin anti-EDG2/LPA1

EDG2/LPA1 Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20442-1-PBS

Synonymes

LPAR1, edg 2, EDG2, Gpcr26, GPR26

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Galerie de données de validation

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  WB analysis of Jurkat using 20442-1-AP (same clone as 20442-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20442-1-AP (EDG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20442-1-PBS in a different storage buffer formulation.

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  WB analysis of multi-cells/tissue using 20442-1-AP (same clone as 20442-1-PBS)

  Multi-cells/tissue were subjected to SDS PAGE followed by western blot with 20442-1-AP (EDG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20442-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 20442-1-AP (same clone as 20442-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20442-1-AP (EDG2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20442-1-PBS in a different storage buffer formulation.

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  WB analysis of A375 using 20442-1-AP (same clone as 20442-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 20442-1-AP (EDG2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20442-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 20442-1-AP (same clone as 20442-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 20442-1-AP (EDG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20442-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 20442-1-AP (same clone as 20442-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20442-1-AP (EDG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20442-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 20442-1-AP (same clone as 20442-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20442-1-AP (EDG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20442-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 20442-1-AP (same clone as 20442-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20442-1-AP (LPAR1,EDG2 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20442-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 20442-1-AP (same clone as 20442-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20442-1-AP (LPAR1,EDG2 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20442-1-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 20442-1-AP (same clone as 20442-1-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug EDG2 Polyclonal antibody (20442-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 20442-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20442-1-PBS cible EDG2/LPA1 dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EDG2/LPA1 Protéine recombinante Ag14253
• Nom complet: lysophosphatidic acid receptor 1
• Masse moléculaire calculée: 364 aa, 41 kDa
• Poids moléculaire observé: 41-50 kDa
• Numéro d’acquisition GenBank: BC030615
• Symbole du gène: EDG2
• Identification du gène (NCBI): 1902
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

EDG2 (also known as LPA1) is a G-protein coupled receptor for lysophosphatidic acid (LPA), a potent motility inducing factor, which is a major component of serum (PMID: 19415462). EDG2 is widely expressed in normal tissue during growth and development. In the context of cancer, several studies have suggested that EDG2 expression in tumors is often similar to that shown in normal tissue (PMID: 17496233).

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