Anticorps Polyclonal de lapin anti-EDIL3

• Phare
• Validé par KD/KO

EDIL3 Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

18

Avis (1)

N° de cat : 12580-1-AP

Synonymes

DEL1, EDIL3

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Galerie de données de validation

• 

  WB analysis using 12580-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12580-1-AP (EDIL3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 12580-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12580-1-AP (EDIL3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12580-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12580-1-AP (EDIL3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12580-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12580-1-AP (EDIL3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12580-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12580-1-AP (EDIL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 12580-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12580-1-AP (EDIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12580-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12580-1-AP (EDIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12580-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12580-1-AP (EDIL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 12580-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12580-1-AP (EDIL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 12580-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12580-1-AP (EDIL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12580-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12580-1-AP (EDIL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12580-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12580-1-AP (EDIL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12580-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12580-1-AP (EDIL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12580-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 12580-1-AP (EDIL3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 12580-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 12580-1-AP (EDIL3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 12580-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 12580-1-AP (EDIL3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules COLO 320, cellules HeLa, cellules HepG2, cellules K-562, tissu cérébral de souris, tissu pulmonaire de souris, tissu pulmonaire humain
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu de cancer du foie humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 8 publications below
• IHC: See 5 publications below
• IF: See 4 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

12580-1-AP cible EDIL3 dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EDIL3 Protéine recombinante Ag3274
• Nom complet: EGF-like repeats and discoidin I-like domains 3
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 52-55 kDa
• Numéro d’acquisition GenBank: BC030828
• Symbole du gène: EDIL3
• Identification du gène (NCBI): 10085
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EGF-like repeats and discoidin I-like domain-containing protein 3 (EDIL3), also named as DEL1 or integrin-biding protein DEL1, is a 52-kDa extracellular matrix protein produced by endothelial cells in embryos (PMID: 9420328). It is composed of three EGF repeats and two discoidin I-like domains. The second EGF repeat contains an RGD motif. EDIL3 promotes adhesion of endothelial cells through interaction with the alpha-v/beta-3 integrin receptor. It may be involved in vascular remodeling during angiogenesis (PMID: 12840057; 14981004). EDIL3 has also been reported to be an endogenous inhibitor of inflammatory cell recruitment by interfering with the integrin LFA-1-dependent leukocyte-endothelial adhesion (PMID: 19008446).

Publications

Species	Application	Title
mouse	IF	J Clin Invest; Secreted protein Del-1 regulates myelopoiesis in the hematopoietic stem cell niche.; Authors - Ioannis Mitroulis ; KO Validated
human	ELISA	Nat Commun; Antagonistic effects of IL-17 and D-resolvins on endothelial Del-1 expression through a GSK-3β-C/EBPβ pathway.; Authors - Tomoki Maekawa
human	WB,IHC	Oncotarget; Overexpressed EDIL3 predicts poor prognosis and promotes anchorage-independent tumor growth in human pancreatic cancer.; Authors - Shu-Heng Jiang ; KD Validated
		Thromb Haemost; Developmental endothelial locus-1 attenuates complement-dependent phagocytosis through inhibition of Mac-1-integrin.; Authors - Ioannis Mitroulis
		Sci Rep; Inhibition of CDK9 as a therapeutic strategy for inflammatory arthritis.; Authors - Annelie Hellvard
human	WB, IHC	Mol Cancer; Elevated autocrine EDIL3 protects hepatocellular carcinoma from anoikis through RGD-mediated integrin activation.; Authors - Ming-Xuan Feng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Guo-rong (Verified Customer) (08-30-2022)

It gives a clear and specific band

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: ARPE-19

EDIL3 Antibody Western Blot validation (1:500 dilution) in ARPE-19 (Cat no:12580-1-AP)

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