Anticorps Recombinant de lapin anti-EDNRB

EDNRB Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

1O2

N° de cat : 82818-2-RR

Synonymes

ET BR, ET B, endothelin receptor type B, Endothelin receptor non-selective type, Endothelin B receptor

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Galerie de données de validation

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  WB analysis using 82818-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82818-2-RR (EDNRB antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  FC experiment of Raji using 82818-2-RR

  1x10^6 Raji cells were intracellularly stained with 0.25 ug EDNRB Recombinant antibody (82818-2-RR, Clone:1O2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu cérébral de rat, tissu cérébral de souris, tissu de cervelet de souris
• Résultats positifs en FC (Intra): cellules Raji,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82818-2-RR cible EDNRB dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: endothelin receptor type B
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: NM_000115
• Symbole du gène: EDNRB
• Identification du gène (NCBI): 1910
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EDNRB (endothelin receptor type B) is a G-protein coupled heptahelical endothelin-1 (EDN1) receptor. EDN1, a known mediator of human myometrial contraction in vitro, belongs to a family of three 21-amino acid isopeptides (PMID:18497345). EDNRB is a 442-residue protein expressed post-natally in many tissues, particularly in the myenteric plexus, mucosal layer, ganglia, and blood vessels of the submucosa of the colon (PMID:11434563). Moreover, Both the EDNRA and EDNRB are expressed in vessels and can be bound by EDN1, but while EDNRA mediates a constrictive effect, EDNRB is involved in vasorelaxation and functions as a clearance receptor that scavenges circulating EDN1 (PMID:30171849).

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