Anticorps Recombinant de lapin anti-EEA1

• Phare
• Validé par KD/KO

EEA1 Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2C11

N° de cat : 81245-1-PBS

Synonymes

ZFYVE2, MSTP105, MST105, Endosome-associated protein p162, Early endosome antigen 1

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Galerie de données de validation

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  WB analysis using 81245-1-RR (same clone as 81245-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81245-1-RR (EEA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81245-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 81245-1-RR (same clone as 81245-1-PBS)

  WB result of EEA1 antibody (81245-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEA1 transfected HepG2 cells. This data was developed using the same antibody clone with 81245-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81245-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81245-1-RR against Human EEA1 were performed. The affinity constant is 0.227 nM.

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Informations sur le produit

81245-1-PBS cible EEA1 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: EEA1 Protéine recombinante Ag28814
• Nom complet: early endosome antigen 1
• Masse moléculaire calculée: 162 kDa
• Poids moléculaire observé: 162 kDa
• Numéro d’acquisition GenBank: NM_003566
• Symbole du gène: EEA1
• Identification du gène (NCBI): 8411
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.

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