Anticorps Monoclonal anti-EEF1A1/EEF1A2

EEF1A1/EEF1A2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4F3F4

N° de cat : 66806-1-PBS

Synonymes

EEF1A2, 4F3F4, eEF1A-2, EEF1AL, EF-1-alpha-2

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Galerie de données de validation

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  WB analysis using 66806-1-Ig (same clone as 66806-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66806-1-Ig (EEF1A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66806-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 66806-1-Ig (same clone as 66806-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66806-1-Ig (EEF1A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66806-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66806-1-Ig (same clone as 66806-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66806-1-Ig (EEF1A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66806-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 66806-1-Ig (same clone as 66806-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66806-1-Ig (EEF1A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66806-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 66806-1-Ig (same clone as 66806-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66806-1-Ig (EEF1A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66806-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 66806-1-Ig (same clone as 66806-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66806-1-Ig (EEF1A2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66806-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66806-1-PBS cible EEF1A1/EEF1A2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: EEF1A1/EEF1A2 Protéine recombinante Ag6875
• Nom complet: eukaryotic translation elongation factor 1 alpha 2
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 48-50 kDa
• Numéro d’acquisition GenBank: BC000432
• Symbole du gène: EEF1A2
• Identification du gène (NCBI): 1917
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

eEF1A2 is one of two isoforms (eEF1A1 and eEF1A2) of eukaryotic elongation factor 1 alpha (eEF1A or eEF1α). The two isoforms share more than 90% sequence identity and have the similar function that being a protein translation factor involved in protein synthesis. Addition, eEF1A2 plays an important role in cell cycle regulation, heat-shock response, aging, posttranslational modifications, and signal transduction (PMID: 25905039). eEF1A2 is expressed in heart, brain tissue, diaphragm and skeletal muscle while eEF1A1 is expressed ubiquitously (PMID:14588074). And the eEF1A2 protein stimulates the phospholipid signaling and activates the Akt-dependent cell migration and actin remodeling that ultimately favors tumorigenesis. It is reported that eEF1A2 was related with kinds of cancers such as ovarian cancer, prostate cancer, pancreatic cancer, breast cancer and lung cancer (PMID:14588074; 24853801; 25744894; 25905039). An 48 kDa band has also been reported(PMID: 24285179).

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