Anticorps Monoclonal anti-EEF1B2

• Phare
• Validé par KD/KO

EEF1B2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3E1C11

N° de cat : 60329-1-PBS

Synonymes

3E1C11, EEF1B, EEF1B1, EF 1 beta, EF1B

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Galerie de données de validation

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  WB analysis using 60329-1-Ig (same clone as 60329-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60329-1-Ig (EEF1B2 antibody) at dilution of 1:2900 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60329-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60329-1-Ig (same clone as 60329-1-PBS)

  WB result of EEF1B2 antibody (60329-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF1B2 transfected HeLa cells. This data was developed using the same antibody clone with 60329-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60329-1-Ig (same clone as 60329-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60329-1-Ig (EEF1B2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60329-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit brain using 60329-1-Ig (same clone as 60329-1-PBS)

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 60329-1-Ig (EEF1B2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60329-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 60329-1-Ig (same clone as 60329-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60329-1-Ig (EEF1B2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60329-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 60329-1-Ig (same clone as 60329-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60329-1-Ig (EEF1B2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60329-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60329-1-Ig (same clone as 60329-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60329-1-Ig (EEF1B2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60329-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 60329-1-Ig (same clone as 60329-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using EEF1B2 antibody (60329-1-Ig, Clone: 3E1C11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60329-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60329-1-PBS cible EEF1B2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: EEF1B2 Protéine recombinante Ag0135
• Nom complet: eukaryotic translation elongation factor 1 beta 2
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 30-34 kDa
• Numéro d’acquisition GenBank: BC000211
• Symbole du gène: EEF1B2
• Identification du gène (NCBI): 1933
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

In eukaryotes, the translation elongation factor eEF1A responsible for transporting amino-acylated tRNA to the ribosome forms a higher-order complex, eEF1H, with its guanine-nucleotide-exchange factor eEF1B. eEF1B consists of three subunits: eEF1B alpha, eEF1B beta and eEF1B gamma. The eEF1B2 possess the nucleotide-exchange activity. Although several models on the basis of in vitro experiments have been proposed for the macromolecular organization of the eEF1H complex, these models differ in various aspects. The human eukaryote elongation factor 1 beta 2 (eEF1B2) migrated as a 30-34 kDa protein in SDS-PAGE.

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