Anticorps Polyclonal de lapin anti-EEF1D

• Phare
• Validé par KD/KO

EEF1D Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(9)

Avis (1)

N° de cat : 10630-1-AP

Synonymes

Elongation factor 1 delta, EF-1-delta, EF1D, EF 1D, EF 1 delta

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Galerie de données de validation

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  WB analysis using 10630-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10630-1-AP (EEF1D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10630-1-AP

  IP result of anti-EEF1D (IP:10630-1-AP, 3ug; Detection:10630-1-AP 1:300) with HeLa cells lysate 1200ug.

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  IP experiment of HeLa using 10630-1-AP

  IP result of anti-EEF1D (IP:10630-1-AP, 3ug; Detection:10630-1-AP 1:300) with HeLa cells lysate 1200ug.

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  IHC staining of human pancreas cancer using 10630-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10630-1-AP (EEF1D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10630-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10630-1-AP (EEF1D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10630-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10630-1-AP (EEF1D antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10630-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10630-1-AP (EEF1D antibody) at dilution of 1:100 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7, cellules MG U-87, cellules NIH/3T3, cellules U2OS
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu cérébral de souris, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

10630-1-AP cible EEF1D dans les applications de WB, IHC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EEF1D Protéine recombinante Ag0983
• Nom complet: eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC007847
• Symbole du gène: EEF1D
• Identification du gène (NCBI): 1936
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EEF1D, also named as EF1D and EF 1 delta, belongs to the EF-1-beta/EF-1-delta family. It is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. EEF1D is phosphorylated upon DNA damage, probably by ATM or ATR. The calculated molecular weight of EEF1D is a 31 kDa, but the modified protein is about 35-40 kDa. ( PMID: 21936567 )

Publications

Species	Application	Title
human	WB	Cell Chem Biol; A photoaffinity labeling strategy identified EF1A1 as a binding protein of cyclic dinucleotide 2'3'-cGAMP.; Authors - Yingjie Hou
human,mouse	WB	EMBO Rep; Transformation of eEF1Bδ into heat-shock response transcription factor by alternative splicing.; Authors - Kaitsuka Taku T ; KD Validated
human	WB	J Exp Clin Cancer Res; EEF1D overexpression promotes osteosarcoma cell proliferation by facilitating Akt-mTOR and Akt-bad signaling.; Authors - Dong-Dong Cheng ; KD Validated
mouse	WB,CoIP	Front Mol Biosci; Heat Shock-Induced Dephosphorylation of Eukaryotic Elongation Factor 1BδL by Protein Phosphatase 1.; Authors - Taku Kaitsuka
mouse	WB	Int J Biochem Cell Biol; The alteration of protein profile induced by cigarette smoking via oxidative stress in mice epididymis.; Authors - Zhu Zijue Z
rat	WB	Proteomics; Pulmonary pressure reduction attenuates expression of proteins identified by lung proteomic profiling in pulmonary hypertensive rats.; Authors - ?stergaard Louise L

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wei (Verified Customer) (03-12-2020)

Sharp and strong band for western blot

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart

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