Anticorps Monoclonal anti-EEF1D

• Phare
• Validé par KD/KO

EEF1D Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

CloneNo.

3B1B11

N° de cat : 60085-1-Ig

Synonymes

3B1B11, Antigen NY-CO-4, EF 1 delta, EF 1D, EF1D

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Galerie de données de validation

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  WB analysis of Jurkat using 60085-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60085-1-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 60085-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60085-1-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 60085-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60085-1-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 60085-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60085-1-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 60085-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60085-1-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 60085-1-Ig

  IP result of anti-EEF1D (IP:60085-1-Ig, 5ug; Detection:60085-1-Ig 1:1000) with MCF-7 cells lysate 3200ug.

• 

  IHC staining of human pancreas cancer using 60085-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60085-1-Ig (EEF1D Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin using 60085-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 60085-1-Ig (EEF1D Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin using 60085-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 60085-1-Ig (EEF1D Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 60085-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 60085-1-Ig(EEF1D antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 60085-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 60085-1-Ig(EEF1D antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 60085-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60085-1-Ig (EEF1D Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 60085-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EEF1D antibody (60085-1-Ig, Clone: 3B1B11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 60085-1-Ig

  Immunofluorescent analysis of Hela cells, using EEF1D antibody 60085-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

• 

  Affinity and Kinetic Characterization of 60085-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 60085-1-Ig against Human EEF1D were performed. The Affinity Constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, cellules MCF-7, cellules NIH/3T3, cellules RAW 264.7
• Résultats positifs en IP: cellules MCF-7,
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu cutané humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

60085-1-Ig cible EEF1D dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: EEF1D Protéine recombinante Ag0983
• Nom complet: eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC007847
• Symbole du gène: EEF1D
• Identification du gène (NCBI): 1936
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EEF1D, also named as EF1D and EF 1 delta, belongs to the EF-1-beta/EF-1-delta family. It is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. EEF1D is phosphorylated upon DNA damage, probably by ATM or ATR. The calculated molecular weight of EEF1D is a 31 kDa, but the modified protein is about 35-40 kDa. ( PMID: 21936567 )

Publications

Species	Application	Title
human	WB	Cell Chem Biol; A photoaffinity labeling strategy identified EF1A1 as a binding protein of cyclic dinucleotide 2'3'-cGAMP.; Authors - Yingjie Hou
mouse	WB	Free Radic Biol Med; Proteomic analysis of the glutathione-deficient LEGSKO mouse lens reveals activation of EMT signaling, loss of lens specific markers, and changes in stress response proteins.; Authors - Jeremy A Whitson
human	WB	Front Microbiol; Strain-Specific Contribution of Eukaryotic Elongation Factor 1 Gamma to the Translation of Influenza A Virus Proteins.; Authors - Shuhei Sammaibashi
human	WB,IF	J Virol; Eukaryotic translation elongation factor 1 delta inhibits the nuclear import of nucleoprotein and PA-PB1 heterodimer of influenza A virus.; Authors - Qingxia Gao ; KO Validated
human	IP	STAR Protoc; Protocol for identification and validation of 2'3'-cGAMP-binding proteins by photoaffinity probes.; Authors - Yingjie Hou
		BMC Biol; Foxg1 regulates translation of neocortical neuronal genes, including the main NMDA receptor subunit gene, Grin1; Authors - Osvaldo Artimagnella

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