Anticorps Polyclonal de lapin anti-EEF1E1

• Phare
• Validé par KD/KO

EEF1E1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 10805-1-AP

Synonymes

AIMP3, EEF1E1, Elongation factor p18, P18

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Galerie de données de validation

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  WB analysis using 10805-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10805-1-AP (EEF1E1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10805-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10805-1-AP (EEF1E1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 10805-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10805-1-AP (EEF1E1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10805-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10805-1-AP (EEF1E1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 10805-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10805-1-AP (EEF1E1 antibody) at dilution of 1:10 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules SKOV-3, tissu testiculaire de rat, tissu testiculaire de souris, tissu testiculaire humain
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

10805-1-AP cible EEF1E1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EEF1E1 Protéine recombinante Ag1092
• Nom complet: eukaryotic translation elongation factor 1 epsilon 1
• Masse moléculaire calculée: 20 kDa
• Poids moléculaire observé: 20 kDa
• Numéro d’acquisition GenBank: BC005291
• Symbole du gène: EEF1E1
• Identification du gène (NCBI): 9521
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

During the translation processes, aminoacyl-tRNA synthetases (ARSs) ligate specific amino acids to tRNAs. Together with non-enzymatic proteins called ARS-interacting multifunctional proteins (AIMPs), the ARSs form a macromolecular complex [PMID:15286174]. This complex has an essential role in protein synthesis, but also in other biological processes including angiogenesis, apoptosis, and inflammation. [PMID:21789020] AIMP3 is not only associated with the multi-tRNA synthetase complex via its specific interaction with methionyl-tRNA synthetase, but also works as a tumor suppressor by interacting with ATM, the upstream kinase of p53 [PMID:18343821].

Publications

Species	Application	Title
human	WB	Mol Cell; A methyltransferase-independent role for METTL1 in tRNA aminoacylation and oncogenic transformation; Authors - Raja H Ali
human	WB	J Cell Mol Med; AIMP3 inhibits cell growth and metastasis of lung adenocarcinoma through activating a miR-96-5p-AIMP3-p53 axis.; Authors - Liting Ding
human	WB	Cell Rep; Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome.; Authors - J J David Ho
human	WB,IF	Proc Natl Acad Sci U S A; Multimodal cotranslational interactions direct assembly of the human multi-tRNA synthetase complex; Authors - Krishnendu Khan ; KD Validated

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