Anticorps Polyclonal de lapin anti-EEF2

• Phare
• Validé par KD/KO

EEF2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20107-1-PBS

Synonymes

EC:3.6.5.-, EF 2, EF2, EF-2, Elongation factor 2

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Galerie de données de validation

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  WB analysis using 20107-1-AP (same clone as 20107-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20107-1-AP (EEF2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 20107-1-AP (same clone as 20107-1-PBS)

  WB result of EEF2 antibody (20107-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF2 transfected HEK-293 cells. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 20107-1-AP (same clone as 20107-1-PBS)

  WB result of EEF2 antibody (20107-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF2 transfected HeLa cells. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 20107-1-AP (same clone as 20107-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20107-1-AP (EEF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 20107-1-AP (same clone as 20107-1-PBS)

  IP result of anti-EEF2 (IP:20107-1-AP, 3ug; Detection:20107-1-AP 1:500) with HeLa cells lysate 1600ug. This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 20107-1-AP (same clone as 20107-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 20107-1-AP (same clone as 20107-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 20107-1-AP (same clone as 20107-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20107-1-AP (EEF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 20107-1-AP (same clone as 20107-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20107-1-AP (EEF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 20107-1-AP (same clone as 20107-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 20107-1-AP (same clone as 20107-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20107-1-AP (EEF2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 20107-1-AP (same clone as 20107-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EEF2 antibody (20107-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 20107-1-AP (same clone as 20107-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human EEF2 (20107-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 20107-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20107-1-PBS cible EEF2 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EEF2 Protéine recombinante Ag13826
• Nom complet: eukaryotic translation elongation factor 2
• Masse moléculaire calculée: 858 aa, 95 kDa
• Poids moléculaire observé: 95-100 kDa
• Numéro d’acquisition GenBank: BC126259
• Symbole du gène: EEF2
• Identification du gène (NCBI): 1938
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

EEF2 (Eukaryotic Elongation Factor 2), also known as EF-2 and EF2, is a crucial protein involved in the process of protein synthesis in eukaryotic cells. It plays a pivotal role in the elongation phase of translation, where it facilitates the movement of the ribosome along the mRNA strand. This movement is essential for the addition of amino acids to the growing polypeptide chain.

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